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KMID : 0377619710210020119
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Korean Jungang Medical Journal
1971 Volume.21 No. 2 p.119 ~ p.128
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S-R form Dissociation of Vibrio cholera and Heavy Metal Ion Reaction
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Abstract
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Various form variants of Vibrio cholerae biotype El Tor strains Ogawa 41 and Hikojima 51 were obtained by culturing the organisms in anti-serum-containing broth.
By routine slide agglutination reaction, colonies grown on plate media were examined as to their mutation degree, using 1% acriflavine solution and homologous immune sera. Five kinds of colony form were chosen according to its intensity of the agglutination reaction and were designated as S-, SSR-, SR-, SSR- and R- form. The agglutination properties of these five colonies , were further investigated by a series of test tube reaction. procedures with various heavy metal ions as well as the acriflavine solution.
Among the reagents tested, lead acetate and acriflavine solutions were most distinguishedly reacted with each of the form variants following its degree of dissociation. When, however, saline was used in stead of distilled water in dissolving the salt and dye, the agglultination titer was considerably modified; the titer of cells with acriflavine solution was generally increased while lowered markedly with lead acetate solution to the degree that the SSR-form, the least dissociated colony, did not show any agglutination. This effect of saline on the lead acetate solution made it possible to differentiate most clearly the SSR-form from the intermediate variant SR-form.
Cobaltous acetate and manganouse chloride solutions presented another characteristic reaction, i.e., even SR-form, as well as SSR-and S-forms, did not agglutinate at all, while SRR- and R-forms reacting intensely. By this reaction, the SR-form was more easily differentiated from the SRR-form.
The agglutination attitude of SRR- and R-forms were similar each other
throughout the tests. The moderately dissociated SRR-form might be, however,differentiated from the extremely dissociated R-form by comparing the reacting intensities in each system.
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